Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

doi:10.1016/S1672-6529(16)60431-6

仿生工程学报 ›› 2017, Vol. 14 ›› Issue (4): 648-658.doi: 10.1016/S1672-6529(16)60431-6

Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

Fedor Senatov1, Natalia Anisimova1,2, Mikhail Kiselevskiy1,2, Aleksey Kopylov2, Viktor Tcherdyntsev1, Aleksey Maksimkin1

1. National University of Science and Technology MISIS, Leninskiy pr. 4, Moscow 119049, Russian Federation
2. N.N. Blokhin Russian Cancer Research Center, Kashirskoye sh. 23, Moscow 115478, Russian Federation

收稿日期:2016-05-17 修回日期:2017-08-17 出版日期:2017-10-10 发布日期:2017-10-10
通讯作者: Fedor Senatov E-mail:senatov@misis.ru
作者简介:Fedor Senatov1, Natalia Anisimova1,2, Mikhail Kiselevskiy1,2, Aleksey Kopylov2, Viktor Tcherdyntsev1, Aleksey Maksimkin1

Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

Fedor Senatov1, Natalia Anisimova1,2, Mikhail Kiselevskiy1,2, Aleksey Kopylov2, Viktor Tcherdyntsev1, Aleksey Maksimkin1

1. National University of Science and Technology MISIS, Leninskiy pr. 4, Moscow 119049, Russian Federation
2. N.N. Blokhin Russian Cancer Research Center, Kashirskoye sh. 23, Moscow 115478, Russian Federation

Received:2016-05-17 Revised:2017-08-17 Online:2017-10-10 Published:2017-10-10
Contact: Fedor Senatov E-mail:senatov@misis.ru
About author:Fedor Senatov1, Natalia Anisimova1,2, Mikhail Kiselevskiy1,2, Aleksey Kopylov2, Viktor Tcherdyntsev1, Aleksey Maksimkin1

摘要/Abstract

摘要： In the present work, Polyhydroxybutyrate (PHB)/Hydroxyapatite (HA) porous composites (10%, 20%, 30 %, 40%, 50% weight HA) were obtained by sintering. PHB/20% HA optimally combines satisfactory mechanical properties with a high content of the bioactive component (HA). Porous PHB/20% HA scaffolds have shown high mechanical properties (compressive strength of 106 MPa and Young’s modulus of 901 MPa). A high volume fraction of interconnected pores (> 50 vol.%) was achieved with pore size of 50 μm – 500 μm. Biocompatibility of porous pure PHB and PHB/20%HA, as its osseointegration were assessed in vitro and after implantation in laboratory animals. PHB/20% HA (–5% ± 0.9%) and pure PHB (–3% ± 1.4%) samples after 24 hours of incubation with human leucocytes showed no significant level of cytotoxicity when p = 0.648 (p-value). In vitro massive adhesion of mouse Multipotent Mesenchymal Stromal Cells (MMSC) to the surface of both porous samples was shown. PHB/20% HA induced more intensive MMSC proliferation compared to pure PHB, which are 31% ± 6.1% and 20% ± 5.7 % respectively when p = 0.039. We observed the resorption (implant surface area was reduced by 49 %) and integration of the porous PHB/20% HA samples into surrounding tissues after 30 days of implantation. The signs of osteoclasts accumulation, neo-angigenesis and new bone formation were observed, which make PHB/20% HA promising for bone tissue engineering.

关键词: hydroxyapatite, scaffold, implant, polyhydroxybutyrate, bone regeneration, porous structure

Abstract: In the present work, Polyhydroxybutyrate (PHB)/Hydroxyapatite (HA) porous composites (10%, 20%, 30 %, 40%, 50% weight HA) were obtained by sintering. PHB/20% HA optimally combines satisfactory mechanical properties with a high content of the bioactive component (HA). Porous PHB/20% HA scaffolds have shown high mechanical properties (compressive strength of 106 MPa and Young’s modulus of 901 MPa). A high volume fraction of interconnected pores (> 50 vol.%) was achieved with pore size of 50 μm – 500 μm. Biocompatibility of porous pure PHB and PHB/20%HA, as its osseointegration were assessed in vitro and after implantation in laboratory animals. PHB/20% HA (–5% ± 0.9%) and pure PHB (–3% ± 1.4%) samples after 24 hours of incubation with human leucocytes showed no significant level of cytotoxicity when p = 0.648 (p-value). In vitro massive adhesion of mouse Multipotent Mesenchymal Stromal Cells (MMSC) to the surface of both porous samples was shown. PHB/20% HA induced more intensive MMSC proliferation compared to pure PHB, which are 31% ± 6.1% and 20% ± 5.7 % respectively when p = 0.039. We observed the resorption (implant surface area was reduced by 49 %) and integration of the porous PHB/20% HA samples into surrounding tissues after 30 days of implantation. The signs of osteoclasts accumulation, neo-angigenesis and new bone formation were observed, which make PHB/20% HA promising for bone tissue engineering.

Key words: polyhydroxybutyrate, hydroxyapatite, porous structure, implant, scaffold, bone regeneration

Fedor Senatov, Natalia Anisimova, Mikhail Kiselevskiy, Aleksey Kopylov . Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts[J]. 仿生工程学报, 2017, 14(4): 648-658.

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Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

Polyhydroxybutyrate/Hydroxyapatite Highly Porous Scaffold for Small Bone Defects Replacement in the Nonload-bearing Parts

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