Journal of Bionic Engineering ›› 2023, Vol. 20 ›› Issue (3): 1049-1059.doi: 10.1007/s42235-022-00317-7
Degradable and Tunable Keratin-fibrinogen Hydrogel as Controlled Release System for Skin Tissue Regeneration
Sung Jun Min1; Jae Seo Lee1; Haram Nah1,2; Ho‑Jin Moon3; Sang Jin Lee3; Hyeon Jeong Kang4; Yu‑Shik Hwang4; Il Keun Kwon3,5; Dong Nyoung Heo2,3
- 1 Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 2 Biofriends Inc, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 3 Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 4 Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea 5 Kyung Hee University Medical Science Research Institute, Kyung Hee University, 23 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea
Degradable and Tunable Keratin-fibrinogen Hydrogel as Controlled Release System for Skin Tissue Regeneration
Sung Jun Min1; Jae Seo Lee1; Haram Nah1,2; Ho‑Jin Moon3; Sang Jin Lee3; Hyeon Jeong Kang4; Yu‑Shik Hwang4; Il Keun Kwon3,5; Dong Nyoung Heo2,3
- 1 Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 2 Biofriends Inc, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 3 Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea 4 Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea 5 Kyung Hee University Medical Science Research Institute, Kyung Hee University, 23 Kyungheedae-Ro, Dongdaemun-Gu, Seoul 02447, Republic of Korea
摘要： Biodegradable hydrogels are promising biomaterials for use in controlled-release systems for skin tissue regeneration. Controlled delivery systems constitute an important aspect of tissue engineering because they can modulate various physiological responses, including early immune response, tissue remodeling, and cell proliferation and maturation in the wound-healing process. Hydrogels composed of various biomaterials have been developed to overcome the limitations of conventional drug- or protein-delivery systems, such as limited targeting ability, low stability, and the induction of drug resistance. Hydrogels based on keratin, a natural polymer extracted from human hair, can provide adequate cell support and control homeostasis. Consequently, they can be applied for skin tissue engineering. In this study, we prepared degradable, tunable, and biocompatible hydrogels for controllable protein delivery. We synthesized keratin-fibrinogen (KER-FBG) by the chemical coupling reaction and prepared hydrogels through polymerization with thrombin. The structures and morphologies of the KER-FBG hydrogels were confirmed. Furthermore, the mechanical properties, swelling ratio, degradation, release behavior, and biocompatibility were investigated. The KER-FBG hydrogels presented promising biological performance, indicating that the material is suitable as a controlled protein delivery carrier.