J4 ›› 2013, Vol. 10 ›› Issue (2): 148-155.doi: 10.1016/S1672-6529(13)60209-7

• 论文 • 上一篇    下一篇

Mingyan Zhao, Lihua Li, Bo Li, Di Wu, Changren Zhou, Ye Tian   

  • 收稿日期:2012-12-10 修回日期:2013-02-10 出版日期:2013-04-06 发布日期:2013-04-10
  • 作者简介: Mingyan Zhao, Lihua Li, Bo Li, Di Wu, Changren Zhou, Ye Tian

A Comparative Study of Fibroblast Behaviors under Cyclic Stress Stimulus and Static Culture on 3D Patterned Matrix

Mingyan Zhao, Lihua Li, Bo Li, Di Wu, Changren Zhou, Ye Tian   

  1. 1. Department of Materials Science and Engineering, Jinan University, Guangzhou 510630, P. R. China
    2. Engineering Research Center of Artificial Organs and Materials (Ministry of Education, China), Guangzhou 510630, P. R. China
    3. Guangdong Medical Devices and Test Institute, Guangzhou 510632, P. R. China
  • Received:2012-12-10 Revised:2013-02-10 Online:2013-04-06 Published:2013-04-10
  • Contact: Lihua Li*, Changren Zhou# E-mail:* tlihuali@jnu.edu.cn, # tcrz9@jnu.edu.cn
  • About author: Mingyan Zhao, Lihua Li, Bo Li, Di Wu, Changren Zhou, Ye Tian

Abstract:

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell func-tionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional (3D) honeycomb patterning Chitosan/Poly(L-Lactic Acid) (CS/PLLA) composites was stimulated by a 15% sinusoidal (1 Hz) strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic Fibroblast Growth Factor (bFGF) secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.

Key words: polymer composites, bFGF, mechanical stimulus, fibroblast