J4 ›› 2014, Vol. 11 ›› Issue (4): 589-599.doi: 10.1016/S1672-6529(14)60070-6

• 论文 • 上一篇    下一篇

Bioactive Titanium Surfaces with the Effect of Inhibiting Biofilm Formation

Chongxia Yue1,2, Bangcheng Yang1,2   

  1. 1. Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, P. R. China
    2. National Engineering Research Center for Biomaterials, Chengdu 610064, P. R. Chin
  • 出版日期:2014-09-30
  • 通讯作者: Bangcheng Yang E-mail:yangbchengc@126.com

Bioactive Titanium Surfaces with the Effect of Inhibiting Biofilm Formation

Chongxia Yue1,2, Bangcheng Yang1,2   

  1. 1. Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, P. R. China
    2. National Engineering Research Center for Biomaterials, Chengdu 610064, P. R. Chin
  • Online:2014-09-30
  • Contact: Bangcheng Yang E-mail:yangbchengc@126.com

摘要:

Titanium metals and its alloy have been widely used in hard tissue repairing fields due to their good biocampatibility and mechanical properties. However, bioinert response and biomaterial associated infections are the main problems for their clinical application. In this study, we chose titanium plates treated with anodic oxidation (AO-Ti), alkali-heat (AH-Ti) and acid-alkali (AA-Ti) methods, which have been proved to be bioactive in vivo, to culture with Staphylococcus aureus and Escherichia coli  to investigate the interaction between bioactive titanium surfaces and biofilm. We used X-ray diffraction (XRD), Scanning Electron Microscope (SEM), roughness measurement to study the physical-chemical properties of the as-received bioactive titanium surfaces, and Confocal Laser Scanning Microscope (CLSM) was employed to study the properties of biofilm formed on the biomaterial surfaces. The results indicate that the titanium surface subjected to anodic oxidation treatment is unfavorable for the formation of biofilm in vitro because the titania (TiO2) coating formed by anodizing has superior antimicrobial property than the other surfaces. Therefore, anodic oxidation surface modification is effective to endow titanium surface with bioactivity and antimicrobial property, which has the potential to improve the successful rate of the clinical application of titanium implants.

关键词: titanium, bioactive, anodic oxidation treatment, antibacterial, biofilm formation

Abstract:

Titanium metals and its alloy have been widely used in hard tissue repairing fields due to their good biocampatibility and mechanical properties. However, bioinert response and biomaterial associated infections are the main problems for their clinical application. In this study, we chose titanium plates treated with anodic oxidation (AO-Ti), alkali-heat (AH-Ti) and acid-alkali (AA-Ti) methods, which have been proved to be bioactive in vivo, to culture with Staphylococcus aureus and Escherichia coli  to investigate the interaction between bioactive titanium surfaces and biofilm. We used X-ray diffraction (XRD), Scanning Electron Microscope (SEM), roughness measurement to study the physical-chemical properties of the as-received bioactive titanium surfaces, and Confocal Laser Scanning Microscope (CLSM) was employed to study the properties of biofilm formed on the biomaterial surfaces. The results indicate that the titanium surface subjected to anodic oxidation treatment is unfavorable for the formation of biofilm in vitro because the titania (TiO2) coating formed by anodizing has superior antimicrobial property than the other surfaces. Therefore, anodic oxidation surface modification is effective to endow titanium surface with bioactivity and antimicrobial property, which has the potential to improve the successful rate of the clinical application of titanium implants.

Key words: titanium, bioactive, anodic oxidation treatment, antibacterial, biofilm formation