J4 ›› 2012, Vol. 9 ›› Issue (4): 470-477.doi: 10.1016/S1672-6529(11)60133-9
The Effect of 58S Bioactive Glass Coating on Polyethylene Terephthalates in Graft-Bone Healing
Yang Wu1, Shiyi Chen1, Jia Jiang1, Hong Li1, Kai Gao1, Pengyun Zhang2
- 1. Department of Sports Medicine and Arthroscopic Surgery, Huashan Hospital, Sports Medicine Center,
Fudan University, Shanghai 200040, P. R. China
2. Shanghai Research Center of Biomedical Engineering, Shanghai 201203, P. R. China
The Effect of 58S Bioactive Glass Coating on Polyethylene Terephthalates in Graft-Bone Healing
Yang Wu1, Shiyi Chen1, Jia Jiang1, Hong Li1, Kai Gao1, Pengyun Zhang2
- 1. Department of Sports Medicine and Arthroscopic Surgery, Huashan Hospital, Sports Medicine Center,
Fudan University, Shanghai 200040, P. R. China
2. Shanghai Research Center of Biomedical Engineering, Shanghai 201203, P. R. China
摘要：
In this study the effects of surface modification of Polyethylene Terephthalates (PET) fibers with 58S bioactive glasses on osteoblasts proliferation and osseointegration in the tibia-articular tendon-bone healing model were investigated. PET sheets were coated with 58S bioactive glass and uncoated PET sheets were used as a control. Scanning Electron Microscope (SEM) and X-ray photoelectron spectrometer were adopted to analyze the surface characteristics of the fibers. MT3T3-E1 cells were cultured with the PET fibers and the MTT and ALP were tested at 1, 3, 5 days. Twenty-four skeletally mature male New Zealand white rabbits were randomly divided into two groups, the 58S-PET group and the PET group. Both groups underwent a surgical procedure to establish a tibia-articular tendon-bone healing model. Mechanical examinations and histological assays were taken to verify the coating effects in vivo. Results of both MTT and ALP tests show significant differences (P < 0.01) between the 58S-PET group and the PET group. At 6 weeks and 12 weeks, the max load-to-failure was significantly higher in the 58S-PET group. In the histological assays, distinct new bone formation was observed only in the 58S-PET group and stronger osseointegration was seen in the 58S-PET group than that in the control group. The 58S-coating on PET could enhance the proliferation and activity of the osteoblasts and therefore promote the new bone formation and tendon-bone healing.