J4 ›› 2011, Vol. 8 ›› Issue (3): 223-233.doi: 10.1016/S1672-6529(11)60029-2

• 论文 • 上一篇    下一篇

Surface Wettability and Chemistry of Ozone Perfusion Processed Porous Collagen Scaffold

Chaozong Liu1, Shirley Z. Shen2, Zhiwu Han3   

  1. 1. School of Mechanical and Systems Engineering, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
    2. Polymer Composite &|Nanomaterials, CSIRO Manufacturing &|Infrastructure Technology, Highett, Vic 3190, Australia
    3. Key Laboratory of Bionic Engineering (Ministry of Education, China), Jilin University, Changchun 130022, P. R. China
  • 出版日期:2011-09-30
  • 通讯作者: Chaozong Liu E-mail:Chaozong.Liu@ncl.ac.uk

Surface Wettability and Chemistry of Ozone Perfusion Processed Porous Collagen Scaffold

Chaozong Liu1, Shirley Z. Shen2, Zhiwu Han3   

  1. 1. School of Mechanical and Systems Engineering, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
    2. Polymer Composite &|Nanomaterials, CSIRO Manufacturing &|Infrastructure Technology, Highett, Vic 3190, Australia
    3. Key Laboratory of Bionic Engineering (Ministry of Education, China), Jilin University, Changchun 130022, P. R. China
  • Online:2011-09-30
  • Contact: Chaozong Liu E-mail:Chaozong.Liu@ncl.ac.uk

摘要:

Crosslinking treatment of collagen has often been used to improve the biological stability and mechanical properties of 3D porous collagen scaffolds. However, accompanying these improvements, the collagen fibril surface becomes hydrophobic nature resulting in a reduced surface wettability. The wetting of the collagen fibril by culture medium is reduced and it is difficult for the medium to diffuse into the 3D structure of a porous collagen scaffold. This paper reports a “perfusion processing” strategy using ozone to improve the surface wettability of chemical crosslinked collagen scaffolds. Surface wettability, surface composition and biological stability were analyzed to evaluate the effectiveness of this surface processing strategy. It was observed that ozone perfusion processing improved surface wettability for both exterior and interior surfaces of the porous 3D collagen scaffold. The improvement in wettability is attributed to the incorporation of oxygen-containing functional groups onto the surface of the collagen fibrils, as confirmed by X-ray Photoelectron Spectroscopy (XPS) analysis. This leads to a significant improvement in water taking capability without compromising the bulk biological stability and mechanical properties, and confirms that ozone perfusion processing is an effective tool to modify the wettability both for interior and exterior surfaces throughout the scaffold.

关键词: scaffold, collagen, surface modification, hydrophilicity, ozone perfusion

Abstract:

Crosslinking treatment of collagen has often been used to improve the biological stability and mechanical properties of 3D porous collagen scaffolds. However, accompanying these improvements, the collagen fibril surface becomes hydrophobic nature resulting in a reduced surface wettability. The wetting of the collagen fibril by culture medium is reduced and it is difficult for the medium to diffuse into the 3D structure of a porous collagen scaffold. This paper reports a “perfusion processing” strategy using ozone to improve the surface wettability of chemical crosslinked collagen scaffolds. Surface wettability, surface composition and biological stability were analyzed to evaluate the effectiveness of this surface processing strategy. It was observed that ozone perfusion processing improved surface wettability for both exterior and interior surfaces of the porous 3D collagen scaffold. The improvement in wettability is attributed to the incorporation of oxygen-containing functional groups onto the surface of the collagen fibrils, as confirmed by X-ray Photoelectron Spectroscopy (XPS) analysis. This leads to a significant improvement in water taking capability without compromising the bulk biological stability and mechanical properties, and confirms that ozone perfusion processing is an effective tool to modify the wettability both for interior and exterior surfaces throughout the scaffold.

Key words: scaffold, collagen, surface modification, hydrophilicity, ozone perfusion