Abstract:

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell func-tionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional (3D) honeycomb patterning Chitosan/Poly(L-Lactic Acid) (CS/PLLA) composites was stimulated by a 15% sinusoidal (1 Hz) strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic Fibroblast Growth Factor (bFGF) secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.

Key words: polymer composites, bFGF, mechanical stimulus, fibroblast