Controlled Cell Patterning on Bioactive Surfaces with Special Wettability
Xiaofeng Zhou1, Jiaqian Li2, Hongyan Sun3, Yi Hu4, Lufeng Che1,5, Zuankai Wang2
- 1. Science and Technology on Microsystem Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
2. Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong 999077, China
3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong 999077, China
4. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multi-disciplinary Research Division, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
5. College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China
Controlled Cell Patterning on Bioactive Surfaces with Special Wettability
Xiaofeng Zhou1, Jiaqian Li2, Hongyan Sun3, Yi Hu4, Lufeng Che1,5, Zuankai Wang2
- 1. Science and Technology on Microsystem Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
2. Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong 999077, China
3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong 999077, China
4. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multi-disciplinary Research Division, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China
5. College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China
摘要： The ability to control cell patterning on artificial substrates with various physicochemical properties is of essence for important implications in cytology and biomedical fields. Despite extensive progress, the ability to control the cell-surface interaction is complicated by the complexity in the physiochemical features of bioactive surfaces. In particular, the manifestation of special wettability rendered by the combination of surface roughness and surface chemistry further enriches the cell-surface interaction. Herein we investigated the cell adhesion behaviors of Circulating Tumor Cells (CTCs) on topographically patterned but chemically homogeneous surfaces. Harnessing the distinctive cell adhesion on surfaces with different topography, we further explored the feasibility of controlled cell patterning using periodic lattices of alternative topographies. We envision that our method provides a designer’s toolbox to manage the extracellular environment.