Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

doi:10.1016/S1672-6529(11)60091-7

摘要/Abstract

摘要：

Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell (hESC) - derived neuronal networks on Microelectrode Array (MEA) platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.

关键词: cell culturing, electrical activity, human embryonic stem cells, microelectrode array, poly(dimethylsiloxane)

Abstract:

Key words: cell culturing, electrical activity, human embryonic stem cells, microelectrode array, poly(dimethylsiloxane)

Joose Kreutzer, Laura Yl?-Outinen, Paula K?rn?, Tiina Kaarela, Jarno Mikkonen, Heli Skottman, Susanna Narkilahti, Pasi Kallio. Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms[J]. J4, 2012, 9(1): 1-10.

参考文献

[1] Goldman S. Stem and progenitor cell-based therapy of the human central nervous system. Nature Biotechnology, 2005, 23, 862–871.

[2] Johnstone A F, Gross G W, Weiss D G, Schroeder O H, Gramowski A, Shafer T J. Microelectrode arrays: A physiologically based neurotoxicity testing platform for the 21st century. Neurotoxicology, 2010, 31, 331–350.

[3] Schmidt C E, Leach J B. Neural tissue engineering: Strategies for repair and regeneration. Annual Review of Biomedical Engineering, 2003, 5, 293–347.

[4] Heikkilä T J, Ylä-Outinen L, Tanskanen J M, Lappalainen R S, Skottman H, Suuronen R, Mikkonen J E, Hyttinen J A, Narkilahti S. Human embryonic stem cell-derived neuronal cells form spontaneously active neuronal networks in vitro. Experimental Neurology, 2009, 218, 109–116.

[5] Ylä-Outinen L, Heikkilä J, Skottman H, Suuronen R, Äänismaa R and Narkilahti S. Human cell-based micro electrode array platform for studying neurotoxicity. Front Neuroeng, 2010, 3, 1–9.

[6] Pine J. Recording action potentials from cultured neurons with extracellular microcircuit electrodes. Journal of Neuroscience Methods, 1980, 2, 19–31.

[7] Thomas C A, Jr., Springer P A, Loeb G E, Berwald-Netter Y, Okun L M. A miniature microelectrode array to monitor the bioelectric activity of cultured cells. Experimental Cell Research, 1972, 74, 61–66.

[8] Gross G W, Rieske E, Kreutzberg G W, Meyer A. A new fixed-array multi-microelectrode system designed for long-term monitoring of extracellular single unit neuronal activity in vitro. Neuroscience Letters, 1977, 6, 101–105.

[9] Erickson J, Tooker A, Tai Y C, Pine J. Caged neuron MEA: A system for long-term investigation of cultured neural network connectivity. Journal of Neuroscience Methods, 2008, 175, 1–16.

[10] Berdichevsky Y, Sabolek H, Levine J B, Staley K J, Yarmush M L. Microfluidics and multielectrode array-compatible organotypic slice culture method. Journal of Neuroscience Methods, 2009, 178, 59–64.

[11] Wagenaar D A, Pine J, Potter S M. An extremely rich repertoire of bursting patterns during the development of cortical cultures. BMC Neuroscience, 2006, 7, 151–156.

[12] Illes S, Fleischer W, Siebler M, Hartung H P, Dihne M. Development and pharmacological modulation of embryonic stem cell-derived neuronal network activity. Experimental Neurology, 2007, 207, 171–176.

[13] van Pelt J, Vajda I, Wolters P S, Corner M A, Ramakers G J. Dynamics and plasticity in developing neuronal networks in vitro. Progress in Brain Research, 2005, 147, 173–188.

[14] van Vliet E, Stoppini L, Balestrino M, Eskes C, Griesinger C, Sobanski T, Whelan M, Hartung T, Coecke S. Electrophysiological recording of re-aggregating brain cell cultures on multi-electrode arrays to detect acute neurotoxic effects. Neurotoxicology, 2007, 28, 1136–1146.

[15] van Kooten T G, Whitesides J F, von Recum A. Influence of silicone (PDMS) surface texture on human skin fibroblast proliferation as determined by cell cycle analysis. Journal of Biomedical Materials Research, 1998, 43, 1–14.

[16] Leclerc E, Sakai Y, Fujii T. Cell culture in 3-dimensional microfluidic structure of PDMS (polydimethylsiloxane). Biomedical Microdevices, 2003, 5, 109–114.

[17] Lee J N, Jiang X, Ryan D, Whitesides G M. Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane). Langmuir, 2004, 20, 11684–11691.

[18] Tourovskaia A, Figueroa-Masot X, Folch A. Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies. Lab Chip, 2005, 5, 14–19.

[19] Rhee S W, Taylor A M, Tu C H, Cribbs D H, Cotman C W, Jeon N L. Patterned cell culture inside microfluidic devices. Lab Chip, 2005, 5, 102–107.

[20] Kim S J, Lee J K, Kim J W, Jung J W, Seo K, Park S B, Roh K H, Lee S R, Hong Y H, Kim S J, Lee Y S, Kim S J, Kang K S. Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells. Journal of Materials Science: Materials in Medicine, 2008, 19, 2953–2962.

[21] Kim Y C, Kang J H, Park S-J, Yoon E-S, Park J-K. Microfluidic biomechanical device for compressive cell stimulation and lysis. Sensors and Actuators B: Chemical, 2007, 128, 108–116.

[22] Dworak B J, Wheeler B C. Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture. Lab Chip, 2009, 9, 404–410.

[23] Park J, Koito H, Li J, Han A. Microfluidic compartmentalized co-culture platform for CNS axon myelination research. Biomedical Microdevices, 2009, 11, 1145–1153.

[24] Teixeira A I, Ilkhanizadeh S, Wigenius J A, Duckworth J K, Inganas O, Hermanson O. The promotion of neuronal maturation on soft substrates. Biomaterials, 2009, 30, 4567–4572.

[25] Adewola A F, Lee D, Harvat T, Mohammed J, Eddington D T, Oberholzer J, Wang Y. Microfluidic perifusion and imaging device for multi-parametric islet function assessment. Biomedical Microdevices, 2010, 12, 409–417.

[26] Liu L, Luo C, Ni X, Wang L, Yamauchi K, Nomura S M, Nakatsuji N, Chen Y. A micro-channel-well system for culture and differentiation of embryonic stem cells on dif-ferent types of substrate. Biomedical Microdevices, 2010, 12, 505–511.

[27] Agastin S, Giang U B, Geng Y, Delouise L A, King M R. Continuously perfused microbubble array for 3D tumor spheroid model. Biomicrofluidics, 2011, 5, 24110.

[28] Khademhosseini A, Ferreira L, Blumling J, 3rd, Yeh J, Karp J M, Fukuda J, Langer R. Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates. Biomaterials, 2006, 27, 5968–5977.

[29] Abhyankar V V, Beebe D J. Spatiotemporal micropatterning of cells on arbitrary substrates. Analytical Chemistry, 2007, 79, 4066–4073.

[30] Gerecht S, Bettinger C J, Zhang Z, Borenstein J T, Vunjak-Novakovic G, Langer R. The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Biomaterials, 2007, 28, 4068–4077.

[31] Ungrin M D, Joshi C, Nica A, Bauwens C, Zandstra P W. Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One, 2008, 3, e1565.

[32] Cimetta E, Figallo E, Cannizzaro C, Elvassore N, Vunjak-Novakovic G. Micro-bioreactor arrays for controlling cellular environments: Design principles for human embryonic stem cell applications. Methods, 2009, 47, 81–89.

[33] Kamei K, Guo S, Yu Z T, Takahashi H, Gschweng E, Suh C, Wang X, Tang J, McLaughlin J, Witte O N, Lee K B, Tseng H R. An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells. Lab Chip, 2009, 9, 555–563.

[34] Korin N, Bransky A, Dinnar U, Levenberg S. Periodic "flow-stop" perfusion microchannel bioreactors for mammalian and human embryonic stem cell long-term culture. Biomedical Microdevices, 2009, 11, 87–94.

[35] Kreutzer J, Lappalainen R S, Ylä-Outinen L, Narkilahti S, Mikkonen J E, Kallio P. Laminin coated PDMS surfaces for long-term measurements of hESC-derived neural networks. Proceedings of the symposium on Microelectrode Arrays in Tissue Engineering, Tampere, Finland, 2009, 23–25.

[36] Serena E, Figallo E, Tandon N, Cannizzaro C, Gerecht S, Elvassore N, Vunjak-Novakovic G. Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. Experimental Cell Research, 2009, 315, 3611–3619.

[37] Villa-Diaz L G, Torisawa Y S, Uchida T, Ding J, Nogueira-de-Souza N C, O'Shea K S, Takayama S, Smith G D. Microfluidic culture of single human embryonic stem cell colonies. Lab Chip, 2009, 9, 1749–1755.

[38] Mata A, Fleischman A J, Roy S. Characterization of polydimethylsiloxane (PDMS) properties for biomedical micro/nanosystems. Biomedical Microdevices, 2005, 7, 281–293.

[39] Merkel T C, Bondar V I, Nagai K, Freeman B D, Pinnau I. Gas sorption, diffusion, and permeation in poly(dimethylsiloxane). Journal of Polymer Science Part B: Polymer Physics, 2000, 38, 415–434.

[40] Shih T-K, Chen C-F, Ho J-R, Chuang F-T. Fabrication of PDMS (polydimethylsiloxane) microlens and diffuser using replica molding. Microelectronic Engineering, 2006, 83, 2499–2503.

[41] Xia Y, Whitesides G M. Soft lithography. Angewandte Chemie International Edition, 1998, 37, 550–575.

[42] McDonald J C, Duffy D C, Anderson J R, Chiu D T, Wu H, Schueller O J, Whitesides G M. Fabrication of microfluidic systems in poly(dimethylsiloxane). Electrophoresis, 2000, 21, 27–40.

[43] Lappalainen R S, Salomaki M, Yla-Outinen L, Heikkila T J, Hyttinen J A, Pihlajamaki H, Suuronen R, Skottman H, Narkilahti S. Similarly derived and cultured hESC lines show variation in their developmental potential towards neuronal cells in long-term culture. Regenerative Medicine, 2010, 5, 749–762.

[44] Potter S M, DeMarse T B. A new approach to neural cell culture for long-term studies. Journal of Neuroscience Methods, 2001, 110, 17–24.