Journal of Bionic Engineering ›› 2024, Vol. 21 ›› Issue (4): 1960-1974.doi: 10.1007/s42235-024-00540-4
Modulated Degradation Rates of Bone Mineral‑Like Calcium Phosphate Glass to Support the Proliferation and Osteogenic Diferentiation of Bone Marrow‑Derived Stem Cells
Lizhe He1,2,3 ; Yuye Huang2 ; Jiafei Gu4 ; Xiaoling Liu4 ; Jun Yin3 ; Xiang Gao1
- 1 Department of Neurosurgery, The First Afliated Hospital of Ningbo University, Ningbo 315010, China 2 Center for Medical and Engineering Innovation, The First Afliated Hospital of Ningbo University, Ningbo 315010, China 3 The State Key Laboratory of Fluid Power Transmission and Control Systems, Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China 4 Faculty of Science and Engineering, University of Nottingham Ningbo China, Ningbo 315100, China
Modulated Degradation Rates of Bone Mineral‑Like Calcium Phosphate Glass to Support the Proliferation and Osteogenic Diferentiation of Bone Marrow‑Derived Stem Cells
Lizhe He1,2,3 ; Yuye Huang2 ; Jiafei Gu4 ; Xiaoling Liu4 ; Jun Yin3 ; Xiang Gao1
- 1 Department of Neurosurgery, The First Afliated Hospital of Ningbo University, Ningbo 315010, China 2 Center for Medical and Engineering Innovation, The First Afliated Hospital of Ningbo University, Ningbo 315010, China 3 The State Key Laboratory of Fluid Power Transmission and Control Systems, Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, China 4 Faculty of Science and Engineering, University of Nottingham Ningbo China, Ningbo 315100, China
摘要： With an elemental composition similar to bone mineral, and the ability to release phosphorus and calcium that beneft bone regeneration, Calcium Phosphate Glass (CPG) serves as a promising component of bone tissue engineering scafolds. However, the degradation of CPG composites typically results in increased acidity, and its impact on bone-forming activity is less studied. In this work, we prepared 3D-printed composite scafolds comprising CPG, Poly-ε-caprolactone (PCL), and various Magnesium Oxide (MgO) contents. Increasing the MgO content efectively suppressed the degradation of CPG, maintaining a physiological pH of the degradation media. While the degradation of CPG/PCL scafolds resulted in upregulated apoptosis of Rat Bone Marrow-derived Stem Cells (rBMSC), scafolds containing MgO were free from these negative impacts, and an optimal MgO content of 1 wt% led to the most pronounced osteogenic diferentiation of rBMSCs. This work demonstrated that the rapid degradation of CPG impaired the renewability of stem cells through the increased acidity of the surrounding media, and MgO efectively modulated the degradation rate of CPG, thus preventing the negative efects of rapid degradation and supporting the proliferation and osteogenic diferentiation of the stem cells.