Preparation and Evaluation of Electrospun Zein/HA Fibers Based on Two Methods of Adding HA Nanoparticles

doi:10.1016/S1672-6529(14)60026-3

Abstract

Abstract:

Zein/HA fibrous membranes were successfully prepared by electrospinning the zein/HA solution mixed by magnetic stirrer (Method I) or ultrasonic power (Method II). The morphology of zein/HA nanocomposite fibers and the distribution of HA within the fibers electrospun by two methods were researched by Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray spectroscopy (EDX). In Method I, the distribution of HA nanoparticles is not homogeneous and HA particles tend to agglom-erate. The relatively homogeneous HA distribution can be observed in the membranes electrospun by Method II. Using mag-netic stirrer to prepare the electrospinning solution improves the wettability of zein/HA membranes. From the viewpoint of application, electrospun zein/HA membranes fabricated by the solution mixed via Methods I and II both possessed reasonable tensile strength and elongation at break for both handling and sterilization. Considering two aspects of strength and elongation, electrospun zein/HA membranes fabricated by Method I are more balanced than those fabricated by Method II. Biological performances of the control zein and zein/HA membranes were assessed by in vitro culture of hMSCs. Results show that both types of the membranes can support cell proliferation. The cells cultured on the zein/HA membranes electrospun by Method I with 5 wt% HA (on weight of zein) show significantly higher proliferation than those cultured on the control zein membranes on the seventh day. The electrospun zein/HA fibrous membranes show promises for bone tissue engineering applications.

Key words: zein/HA fibers, MSCs, electrospinning, cell proliferation, tissue engineering

Mei Zhang, Yongjia Liu, Yuqing Jia, Hongshuang Han, Dahui Sun. Preparation and Evaluation of Electrospun Zein/HA Fibers Based on Two Methods of Adding HA Nanoparticles[J].J4, 2014, 11(1): 115-124.

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