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Using a Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Chicken Carcass

Yu-bin Lan1; Shi-zhou Wang2; Yong-guang Yin3; W. Clint Hoffmann1; Xian-zhe Zheng4   

  1. 1. USDA-ARS, College Station, TX 77845, USA
    2. Department of Environmental and Occupational Health, Texas A&M University, College Station, TX 77845, USA
    3. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, P. R. China
    4. College of Engineering, Northeast Agricultural University, Harbin 150030, P. R. China
  • Received:2008-04-17 Revised:2008-06-17 Online:2008-09-30 Published:2008-06-17
  • Contact: Yu-bin Lan

Abstract: Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodborne pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically “taste” the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif-ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con-centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 × 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.

Key words: Salmonella Typhimurium, biosensor, food safety, chicken carcass, detection limitation, Surface Plasmon Resonance