Abstract: 3D-printed Porous Titanium Alloy Implants (pTi), owing to their biologically inertness and relatively smooth surface
morphology, adversely affect the biological functions of surrounding cells. To address the challenges, constructing a bioinspired
interface that mimics the hierarchical structure of bone tissue can enhance the cellular functions of cells. In this
context, Hollow Mesoporous Silica Nanoparticles (HMSNs), renowned for their unique physicochemical properties and
superior biocompatibility, offer a promising direction for this research. In this research, the initially synthesized HMSNs
were used to construct a “hollow-mesoporous-macroporous” hierarchical bioinspired coating on the pTi surface through
the Layer-by-Layer technique. Simultaneously, diverse morphologies of coatings were established by adjusting the deposition
strategy of PDDA/HMSNs on the pTi surface (pTi-HMSN-2, pTi-HMSN-4, pTi-HMSN-6). A range of techniques
were employed to investigate the physicochemical properties and regulation of cellular biological functions of the diverse
HMSN coating strategies. Notably, the pTi-HMSN-4 and pTi-HMSN-6 groups exhibited the uniform coatings, leading
to a substantial enhancement in surface roughness and hydrophilicity. Meantime, the coating constructed strategy of pTi-
HMSN-4 possessed commendable stability. Based on the aforementioned findings, both pTi-HMSN-4 and pTi-HMSN-6
facilitated the adhesion, spreading, and pseudopodia extension of BMSCs, which led to a notable upsurge in the expression
levels of vinculin protein in BMSCs. Comprehensive analysis indicates that the coating, when PDDA/HMSNs are
deposited four times, possesses favorable overall performance. The research will provide a solid theoretical basis for the
translation of HMSN bioinspired coatings for orthopedic implants.

Key words: Hollow mesoporous silica nanoparticles · Bioinspired coating · Titanium alloy · Hierarchical structure · , Cellular functions