Journal of Bionic Engineering ›› 2023, Vol. 20 ›› Issue (4): 1635-1645.doi: 10.1007/s42235-023-00355-9

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Development of Bio-cultured Artificial Muscles with High Design Flexibility

Hirono Ohashi1; Shunsuke Shigaki1; Ryo Teramae1; Masahiro Shimizu1; Koh Hosoda1   

  1. 1 Department of Systems Innovation, Osaka University, 1-2, Machikaneyama, Toyonaka, Osaka 560-0043, Japan
  • 出版日期:2023-07-10 发布日期:2023-07-10
  • 通讯作者: Hirono Ohashi E-mail:ohashi@arl.sys.es.osaka-u.ac.jp
  • 作者简介:Hirono Ohashi1; Shunsuke Shigaki1; Ryo Teramae1; Masahiro Shimizu1; Koh Hosoda1

Development of Bio-cultured Artificial Muscles with High Design Flexibility

Hirono Ohashi1; Shunsuke Shigaki1; Ryo Teramae1; Masahiro Shimizu1; Koh Hosoda1   

  1. 1 Department of Systems Innovation, Osaka University, 1-2, Machikaneyama, Toyonaka, Osaka 560-0043, Japan
  • Online:2023-07-10 Published:2023-07-10
  • Contact: Hirono Ohashi E-mail:ohashi@arl.sys.es.osaka-u.ac.jp
  • About author:Hirono Ohashi1; Shunsuke Shigaki1; Ryo Teramae1; Masahiro Shimizu1; Koh Hosoda1

摘要: Recent advances in bionics have made it possible to create various tissue and organs. Using this cell culture technology, engineers have developed a robot driven by three-dimensional cultured muscle cells (bioactuator)—a muscle cell robot. For more applications, researchers have been developed various tissues and organs with bio3D printer. However, three-dimensional cultured muscle cells printed by bio3D printer have been not used for muscle cell robot yet. The aim of our study is to develop easy fabrication method of bioactuator having high design flexibility like as bio3D printer. We fabricated three-dimensional cultured muscle cells using mold and dish having pin which can contribute to shape and cell alignment. In this study, we observed that our method maintained the shape of three-dimensional cultured muscle cells and caused cell alignment which is important for bioactuator development. We named three-dimensional cultured muscle cells developed in this study “bio-cultured artificial muscle (BiCAM)”. Finally, we observed that BiCAM contracted in response to electrical stimulus. From these data, we concluded our proposed method is easy fabrication method of bioactuator having high design flexibility.

关键词: Three-dimensional cultured muscle cells , · Bio-cultured artificial muscle , · Skeletal muscle cell , · Tissue engineering

Abstract: Recent advances in bionics have made it possible to create various tissue and organs. Using this cell culture technology, engineers have developed a robot driven by three-dimensional cultured muscle cells (bioactuator)—a muscle cell robot. For more applications, researchers have been developed various tissues and organs with bio3D printer. However, three-dimensional cultured muscle cells printed by bio3D printer have been not used for muscle cell robot yet. The aim of our study is to develop easy fabrication method of bioactuator having high design flexibility like as bio3D printer. We fabricated three-dimensional cultured muscle cells using mold and dish having pin which can contribute to shape and cell alignment. In this study, we observed that our method maintained the shape of three-dimensional cultured muscle cells and caused cell alignment which is important for bioactuator development. We named three-dimensional cultured muscle cells developed in this study “bio-cultured artificial muscle (BiCAM)”. Finally, we observed that BiCAM contracted in response to electrical stimulus. From these data, we concluded our proposed method is easy fabrication method of bioactuator having high design flexibility.

Key words: Three-dimensional cultured muscle cells , · Bio-cultured artificial muscle , · Skeletal muscle cell , · Tissue engineering